Effects of Double Layer Centrifugation on the Improvement of Sperm Quality in Dogs: A Comparative Note among Different Breeds

The evaluation of sperm quality in the laboratory is essential to improve efficiency assisted reproduction. As other species, for dog there are reports that prolonged contact with some components seminal plasma associated decreased motility and viability. Thus, centrifugation a technique widely used concentrate spermatozoa eliminate supernatant. purpose this study was evaluate effect double layer on percentages total progressive dog’s semen submitted dilution, single centrifugation, cooling storage at 5 °C 24 48 hours. For purpose, ejaculates 30 healthy male dogs were evaluated, by taking into account comparison among conventional parameters (ejaculate volume, concentration, motility). samples examined standard baseline condition fresh (FS), after dilution (AD), (SLC), (DLC). According different time points, evaluated conditions, immediately their collection (T0), h (T24) (T48), treatments semen’s quality. Results showed significant improvement dogs. use soon will up 48h, special emphasis motility, adding an alternative technical approach reproductive performance breeding